Ficus microcarpa fruit derived iron oxide nanomaterials and its anti-bacterial, antioxidant and anticancer efficacy

14^th International Virology Summit & 9^th International Conference on Gynecology and Obstetrics

August 22, 2022 Barcelona, Spain | Hybrid Event

Fuad Abdullah Alatawi

University of Tabuk, Saudi Arabia

Posters & Accepted Abstracts: J AIDS Clin Res

Abstract :

Microbial infections-based diseases are a significant public health issue around the world, mainly when antibiotic-resistant bacterium types evolve. In this research, we explored the anti-bacterial and anticancer potency of iron-oxide (Fe2O3) nanoparticles prepared from F. macrocarpa fruit extract. The chemical composition of F. macrocarpa fruit extract was used as reducing and capping agent for nanoparticles’ synthesis was examined by GC-MS/MS analysis. Then, the prepared nanoparticles were confirmed by various biophysical techniques, including X-Ray Powder Diffraction (XRD), Fourier-Transform Infrared Spectroscopy (FTIR), UV-Vis Spectroscopy, Transmission Electron Microscopy (TEM) and Energy Dispersive Spectroscopy (EDAX), and Dynamic Light Scattering (DLS). Also, the antioxidant capacity of fruit extract was determined through 2,2-diphenyl-1-picrylhydrazyl (DPPH), 2,2'-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid (ABTS), Fluorescence Recovery After Photobleaching (FRAP), Superoxide Dismutase (SOD) assays. Furthermore, the cytotoxicity activities of Fe2O3 NPs were determined using the MTT test on MCF-7 cells. In antibacterial assay, lethal doses of the Fe2O3 NPs effectively inhibited the growth of gram-negative, grampositive bacteria. The surface damage, ROS production, and protein leakage are the antibacterial mechanisms of Fe2O3 NPs. Concerning antioxidant activity, the fruit extracts of F. microcarpa had a strong antioxidant property, which was confirmed by DPPH, ABTS, FRAP, and SOD assays. In addition, the F. microcarpa derived iron oxide nanomaterials greatly reduced the cell viability on (MCF-7). The GC-MS/MS analysis revealed the presence of 25 main bioactive compounds in the F. microcarpa extract. Overall, the finding of this research revealed that F. microcarpa derived Fe2O3 nanoparticles could be employed as an alternative therapeutic agent to cure microbial infection and breast cancer in humans. Keywords: Ficus microcarpa; Iron oxide; Antibacterial activity; Cytotoxicity.