Formulation and development of liquid rabies vaccine for human use with trehalose as a stabilizer

R Vijayakumar

: J Bioengineer & Biomedical Sci

Abstract :

Rabies is normally a rapidly fatal neurological disease and to date therapeutic efforts in humans have proved futile except in rare cases in which rabies vaccine was administered prior to the onset of clinical disease. One of the most elements in the effective control of human rabies is the use of efficacious vaccines. According to the survey conducted by WHO across different parts of the globe, every year 50,000 of peoples die because of rabies. Rabies is transmitted by the saliva of the infected animals such as Dogs, Fox, Wolf, Bats etc. There are many vaccines developed and are available for the rabies treatment across the world. The Vaccines are of different types such as, Nervous Tissue Vaccine, VERO Cell Line based Vaccine, Human Diploid Cell Vaccine, and Chick Embryo based Vaccines.Our study is a preliminary approach is to develop the liquid state rabies vaccines with Trehalose, Human Albumin and Aluminium Phosphate as a stabilizer, additive and adjuvant respectively. Before formulation the purified VERO cell line derived virus PV 11 (Purified Virus) was concentrated, infected and chromatographically purified. The rabies antigens efficacy, potency are checked by invitro methods like PN2, Single Radial Immuno Diffusion Assay, protein content 260/280, Total Protein content and by BCA method. Then the PV 11 was formulated and it was labelled as TCALRV (Tissue Culture Anti-Liquid Rabies Vaccine) and the further studies of the vaccine are in progress.

Biography :

Mr. R. Vijayakumar has completed his M.Sc in Biochemistry in his 22 years in Karpagam Arts and Science College (Presently Karpagam University) and B.Sc in MIET Arts and Science College, Trichirapalli, Tamilnadu. He is presently working as a Junior Biochemist in M. V. Hospital for Diabetes and Diabetes Research Centre, Royapuram, Chennai, India. He is working on the stabilization of rabies vaccine. He has published 2 papers in reputed journals.