Ganoderma lucidum-cytotoxic effects and cytokine gene induction as compared with the immunobladder® BCG in murine MB49 cells

6^th World Congress on Medicinal Chemistry and Drug Design

June 07-08, 2017 Milan, Italy

John W M Yuen, Cyrus K C Ho, Tony S S To and Chi-Fai Ng

The Hong Kong Polytechnic University, Hong Kong
The Chinese University of Hong Kong, Hong Kong

Scientific Tracks Abstracts: Med Chem (Los Angeles)

Abstract :

Immunotherapeutic effects of the ethanol extract of Ganoderma lucidum (GLe) were compared against the conventional immunobladder�® Bacillus Calmette-Gu�©rin (BCG) in terms of cytotoxicity, cell cycle analysis and cytokine genes expression, in vitro. In conjunction with the intravesical study using the orthotopic MB49/C57 mice model, the murine urothelial carcinoma MB49 cell line was used for experiments. In agreement with the previous findings, GLe was demonstrated to exhibit G2/M phase cell arrest. On the other hand, dose-dependent cytotoxicity was demonstrated by both GLe and BCG as measured by the lactate dehydrogenase (LDH) assay; however, GLe concentrations ranged from 40 to 100 �¼g/ml killed 24.7-88.1% of the MB49 cells, which was superior to the 250-1000 �¼g/ml of BCG that killed 7.6-19.6%. Such cytotoxic effects were also shown to be inter-correlated with the expression of several cytokine genes, which are known to be important for anticancer. Although both GLe and BCG were shown to be active in inducing the interleukin(IL)-6, IL-12b and interferon-gamma (IFN-�³), dose-dependent inductions were only demonstrated by the range of GLe concentrations being tested. Particularly, the induction of IFN-�³ gene was denominated by GLe up to 4-folded, as compared with the 1.5-folded increase by BCG. Basic research on immunobladder�® BCG is limited and given that IFN-�³ is wellevidenced for its anticancer effects, results herein speculated GLe could be an immunotherapeutic agent superior to the BCG by exerting stronger cytotoxic effects via a pathway involving IFN-�³ and other molecules. The in vivo effects of GLe are currently being examined in animals.

Biography :

John W M Yuen is currently an Associate Professor from the School of Nursing of the Hong Kong Polytechnic University. He is a biomedical scientist who has completed his PhD in 2007, with a focus on cancer and immunology in the field of Urology. His research team conducts different types of research by adopting a wide range of methodologies from exploratory cross-sectional/cohort design and in vitro laboratory experiments to in vivo trails on animals and humans.

Email: john.yuen@polyu.edu.hk