Genome structure sharing by reptiles and avians as of Isospora from bearded dragon and black throated laughing thrush

8^th International Conference on Animal Health & Veterinary Medicine

October 20-21, 2017 | Toronto, Canada

Mian A Hafeez and John R Barta

University of Guelph, Canada
University of Veterinary and Animals Sciences, Lahore, Pakistan

Scientific Tracks Abstracts: J Vet Sci Technol

Abstract :

The complete mitochondrial genome of Isospora amphiboluri and Isospora sp. n. (Eimeriidae, Coccidia, Apicomplexa) were sequenced. These coccidia infect the bearded dragon, Pogona vitticeps (Sauria: Agamidae) and black throated laughing thrush Garrulax chinensis (Aves: Passeriformes) respectively. PCR was performed post DNA extraction and protein estimation by Nanodrop. The Whole Genome was achieved in fragments and assembled. Genome organization and gene content was conventional. The circular-mapping mt genome of Isospora amphiboluri is 6264bp and Isospora sp. n. 6158bp in length consisting of 3 protein coding genes (cox1, cox3 and cytb), 19 gene fragments encoding large subunit (LSU) rRNA and 14 gene fragments encoding small subunit (SSU) rRNA. Like other Apicomplexan, no tRNA was encoded. The comparable mitochondrial genome sequences and structures of Isospora and Eimeria species confirm the close relationship between these eimeriid genera of apicomplexan parasites. Investigation of Eimeria genes intricate the basic biology and host-parasite interaction and highlights adaptations to a comparatively simple developmental life cycle. The purpose of this study was to sequence and annotate the complete mitochondrial genomes of Isospora amphiboluri and Isospora sp. n that commonly infect bearded dragon and black throated laughing thrush.

Biography :

Mian A Hafeez has completed his Doctor of Veterinary Medicine (DVM), MPhil and his PhD from University of Agriculture, Faisalabad, Pakistan. He did his Post-doctoral studies from University of Guelph, Ontario Veterinary College, Canada and continued working for seven years as Diagnostic Molecular Parasitologist. Currently, he is working as Associate Professor (visiting, on leave from Ontario Veterinary College) in University of Veterinary & Animal Sciences, Lahore, Pakistan. He has published more than 35 papers in reputed journals and has been serving as an Editorial Board Member of repute.