Genotoxic/anti-genotoxic activities of Clematis flammula extracts

Atmani Dina; Tahiri Ouahiba; Debbache-Benaida Nadjet; Ayouni Karima; Berboucha Meriem; Saidene Naima; Charid Imene; Frederic Amant; Stefanie Schrauwen

doi:10.4172/2167-7689-C1-025

Genotoxic/anti-genotoxic activities of Clematis flammula extracts

9^th Annual European Pharma Congress

June 26-28, 2017 Madrid, Spain

Atmani Dina, Tahiri Ouahiba, Debbache-Benaida Nadjet, Ayouni Karima, Berboucha Meriem, Saidene Naima, Charid Imene, Frederic Amant and Stefanie Schrauwen

Universit�?© de Bejaia, Algeria
University of Leuven, Belgium
University Hospital Leuven, Belgium
Antoni van Leeuwenhoek-Netherlands Cancer Institute, Netherlands

Posters & Accepted Abstracts: Pharmaceut Reg Affairs

Abstract :

Clematis flammula leaf extracts are widely used in folklore medicine in Algeria to treat anti-inflammatory disorders and anticancer potential. Validation of the use of medicinal plants should also shed the light on their safety, based on the lack of their cytotoxicity and genotoxicity. The aim of our study was to assess the cytotoxicity and genotoxicity/anti-genotoxicity of the plant leaf extracts by the Allium cepa root test. In the same context, we tested their anticancer potential on two ovarian cancer cell lines OVCAR3 and A2780. Morphological observations of Allium cepa root cells after treatment by 100 and 300 �?¼g/kg of C. flammula leaf extracts, sodium azide and a mixture of both have revealed that an absence of toxicity was observed for the plant extracts contrary to sodium azide. However, the combination of C. flammula extract at 300 �?¼g/ml with sodium azide has induced a shortening of the root bulb (�?�?L between â�?�? 1.22 mm and 0.02 mm) associated with marked changes in color, form, and consistency. Similarly, the mitotic index (MI) was impacted by sodium azide (100 �?¼g/ml) especially in prophase but not with the extract (100, 300 �?¼g/ml). The results are confirmed by the increase of chromosomal aberrations (C-mitosis, anaphase bridges and micronuclei) following sodium azide treatment. On the other hand, the MTT test indicated that survival of ovarian cancer cells (OVCAR3) was reduced to half at 10 �?¼g/ml after 72 h which was less effective than that against A2780 of which survival was reduced to almost 30% at the same concentration and time scale. Bioactive compounds were identified by HPLC-MS.