Genotype induced behavior of perivascular mesenchymal stem cells (pericytes) in vitro

5^th International Conference on Tissue Engineering & Regenerative Medicine

September 12-14, 2016 Berlin, Germany

Sudheer Shenoy P

Yenepoya University, India

Scientific Tracks Abstracts: J Tissue Sci Eng

Abstract :

Pericytes are a source of mesenchymal stem cells (MSCs) having multilineage differentiation potential that are found on the wall of blood vessels. We have isolated, purified and characterized pericytes from liver as CD146+CD34-CD45-CD56- from wild-type (WT) and myostatin null (Mstn-/-) mice. CD146+ cells isolated from WT liver expressed myostatin and pericytes from both the genotypes expressed pericyte and adult stem cell markers and did not express �±SMA and GFAP. CD146+ cells could be readily differentiated into adipogenic, osteogenic and chondrogenic lineages. When subjected to myogenic differentiation, these CD146+ cells behaved contrastingly as fibrogenic and myogenic precursors when isolated from two different genotypes WT and Mstn-/- respectively. Presence or absence of myostatin in vitro may play a role in determining the fate of pericytes like cells in liver. Furthermore CD146+ cells from the liver can be useful candidates for cell therapy studies.

Biography :

Sudheer Shenoy P holds a PhD degree in Biochemistry from Kuvempu University, India in 2005. He has about 20 years of pre and post-PhD research experience in Tissue Engineering and Stem Cell Biology in India and Singapore. He holds key skills in the field of Generating Clinical, as well as, R&D grade Stem Cells. He has several international publications and 3 patents to his credit. His present focus is basic biology of adult perivascular mesenchymal stem cells and also various aspects of its application in cell therapy.

Email: shenoy2000@yahoo.com