Genotypic and phenotypic characterization of methicillin resistant staphylococcus aureus in Kiambu county Kenya

Joint Event on 12^th International Conference on Genomics and Molecular Biology & 12^th European Biosimilars Congress

April 15-17, 2019 Berlin, Germany

Sani Iliya

Mount Kenya University, Kenya

Posters & Accepted Abstracts: J Bioanal Biomed

Abstract :

Methicillin resistant Staphylococcus aureus causes illness to people in both healthcare facilities and surrounding environment leading to high rate of morbidity and mortality. Resistance to S. aureus is mainly caused by resistance gene PBP2a found in antibiotic with β-lactam antibiotics such as penicillins, cephalosporins and carbapenems. Samples from wound swabs were collected from patients attending Kiambu and Thika Level-5 hospitals. Out of 138 samples, 54 (39.1%) were culture positive for S. aureus. There was no significant association between S. aureus prevalence and the gender of patients from the study (Chi Square = 3.00; degree of freedom = 1; p = 0.08). Twenty two (22) samples tested positive for MRSA based on Cefoxitin susceptibility test, indicating a prevalence of 15.9% (22/138; 95% CI = 9.4 - 22.1). Susceptibility to 9 common antibiotics ranged from 15% to 85% in the 54 isolates. A total of 27 isolates with MRSA infection were obtained based on the genotypic analysis which is a prevalence of 19.6% (27/138; 95% CI = 13.8-25.4). Out of the 54 S. aureus isolates, 14 (25.9%; 95% CI = 11.9- 38.9) had mecA gene and 14 (25.9%; 95% CI = 11.9-38.1) had Panton-Valentine Leucocidin (PVL) genes. DNA sequencing revealed 18 different spa types circulating in the study area with the most frequent being t355 at 50%. More data on these findings, which have a huge impact on the rational use of antibiotics in Kenya, will be presented.

Biography :

Email: saliyasu@yahoo.com