Gomisin N inhibits adipogenesis and prevents high-fat diet-induced obesity

8^th International Conference on Natural & Alternative Medicine

September 25-27, 2017 Dubai, UAE

Myeong Ho Jung

Pusan National University, South Korea

Posters & Accepted Abstracts: Altern Integr Med

Abstract :

Gomisin N (GN) is a physiological lignan derived from Schisandra chinensis. In the present study, we investigated the inhibitory effects of GN on differentiation of 3T3-L1 preadipocytes and the anti-obesity effects of GN in high-fat diet (HFD)-induced obese mice. Incubation with GN significantly inhibited the differentiation of 3T3-L1 preadipocytes in a dosedependent manner. This inhibitory effect primarily occurred at an early adipogenic stage through impairment of mitotic clonal expansion (MCE) caused by cell cycle arrest at the G1/S phase transition. GN inhibited the extracellular signal-regulated kinase and phosphoinositide 3-kinase/protein kinase B signaling in the MCE process and activated AMP-activated protein kinase. Furthermore, GN down-regulated CCAT/enhancer-binding protein �² (C/EBP�²) and histone H3K9 demethylase JMJD2B during early stages of adipogenesis and therefore repressed the expression of C/EBP�²-targeted cell cycle genes. In addition, GN also repressed the expression of histone H3K4 methyltransferase MLL4 and reduced PPAR�³ expression. Moreover, GN effectively lowered the final body weight, adipose tissue mass and reduced the serum levels of glucose, total triglyceride and cholesterol in the HFD-induced obese mice. GN also markedly reduced hepatic triglyceride level induced by HFD. Collectively, these findings suggest that GN has potential as a novel agent for the prevention and treatment of obesity.