Green synthesis of phytochemical-stabilized nontoxic silver nanoparticles and their chemopreventive potential

4^th Global Acupuncture & Therapists Annual Meeting and International Conference on Holistic Medicine & Holistic Nursing

July 14-16, 2016 Philadelphia, USA

Debjani Nath and Pratyusha Banerjee

University of Kalyani, India

Scientific Tracks Abstracts: Altern Integr Med

Abstract :

Green chemistry methods offer opportunities to design greener production technologies with safer nanomaterial, reduced environmental impact, waste reduction and energy efficiency. Silver nanomaterials are one of the most commonly utilized nanomaterials interest because of its potential biomedical applications. In this study an attempt has been made for phytofabrication of silver nanoparticles by reducing silver with aqueous bark extract of Saraca asoca (Roxb.) (Belonging to family Caesalpiniaceae) having high concentration of flavonoids, terpenoids, saponins, phenol, tannin, lignin, cardiac glycosides and proteins. The properties of these green-Au NPs were characterized by TEM, UV/Vis and FT-IR spectroscopy and the Au NPs exhibited excellent homogeneity with an average diameter of 3-10 nm and high dispersity at all pH ranges with long-term stability as well as excellent cytocompatibility. FT-IR analysis of surface property of fabricated nanomaterial and GCMS analysis of extracted phytochemical stabilizers indicated the involvement of carboxyl (-C= O), hydroxyl (-OH) and amine (-NH) functional groups of the phytochemicals present in S. asoca bark extract in reducing, capping and stabilizing silver nanoparticles. The rapid electrokinetic behaviour of the silver was evaluated using zetapotential (approx -23.2 mV), which was highly dependent on nanoparticle acidity and particle size. The potential toxicological effect of these particles has been studied using in vitro cytotoxicity analysis as well as in vivo mouse model. No significant level of toxicity was observed at highest dose of 5000 mg/Kg body weight. The chemopreventive properties of the phytofabricated nanomaterial were significant as analysed using benzene exposed secondary acute myelolytic leukemia mouse model. This novel synthesis route for Au NPs using plant extract reducing agent may be effectively exploited for nontoxic energy efficient nanomaterial for medical applications.

Biography :

Debjani Nath has completed her PhD from Jadavpur University, India and Post-doctoral studies from Indian Institute of Chemical Biology, a unit of Council of Scientific and Industrial Research, India. She is Professor of Zoology in University Of Kalyani, Nadia, West Bengal, India. She has published more than 40 papers in reputed journals and has been serving as an Editorial Board Member of repute.

Email: nath_debjani@yahoo.co.in