Hair follicle regeneration from human pluripotent stem cells

12^th International Conference and Exhibition on Cosmetic Dermatology and Hair Care

November 28-30, 2016 San Antonio, USA

Michel R Hanna, Yang Liu, Hasan El-Fakahany, Walid Medhat, Hamza Abdel-Raouf and Evan Y Snyder

Minia University, Egypt
Sanford-Burnham-Prebys Medical Discovery Institute, USA

Posters & Accepted Abstracts: J Cosmo Trichol

Abstract :

Hair follicle (HF) morphogenesis and regeneration depend on intensive and reciprocal interactions between epithelial and mesenchymal components. Currently, attempts to regenerate HFs depend on combining receptive epithelial and trichogenic dermal mesenchymal components and grafting them into an in vivo environment. Unfortunately, human HF bulge stem cells (BSCs) are not suitable for this purpose because the isolation and propagation of human HF BSCs for tissue engineering purposes remains a challenge. Here, we developed a strategy to differentiate human embryonic stem cells (hESCs) and induced pluripotent stem cells (hiPSCs) into CD200+/ITGA6+ BSCs that can reconstitute the epithelial components of the HF. Importantly, co-transplantation of hESCs or hiPSC-derived CD200+/ITGA6+ cells with trichogenic mice dermal cells into immunodeficient Nude mice resulted in HF formation. Histological analysis revealed that the obtained HFs possess all HF lineages including the hair shaft, and the inner and outer root sheaths. Human HF stem cell markers such as keratin-15 were detected in reconstituted HFs. The human origin of the epithelial cells in the new HFs was confirmed by positive reactivity for human-specific nuclear antigen. In this context derivation of functional HF BSCs capable of inducing a new hair formation suggest a major step toward developing cell-based treatments for alopecia.

Biography :

Email: mibrahim@sbpdiscovery.org