Heat acclimation associated with decreased of urinary 8-hydroxydeoxyguanosine in navy boiler tender

2^nd World Congress on Biomarkers & Clinical Research

12-14 September 2011 Baltimore, USA

Yung-Kai Huang, Chen-Chen Chang, Che-Yi Lai, Hsin-Hsiu Huang and Horn-Che Chiang

Scientific Tracks Abstracts: J Mol Biomark Diagn

Abstract :

The physiological effects of heat are reviewed extensively elsewhere, but there is signifi cant and poorly documented on heat tolerance in occupational exposure. Pervious studies show that the hyperthermia has been induced reactive oxygen species and DNA damage. Th e aim of this study was to elucidate the association between heat acclimatization or body composition and urinary 8-hydroxydeoxyguanosine (U-8OHdG) level in navy boiler tender. Data collections including a standardized questionnaire four repeated-measures (pre- sailing, aft er-sailing, per-work shift , and post-work shift ) of urine sample were performed to measure the level of U-8OHdG and urinary electrolytes. Th e body composition of workers was provided by the body composition analyzer. Th e ion of sodium, potassium, and chlorine in urine and sweat was measured by electrolytic analyzer. U-8OHdG was measured by liquid chromatography with tandem mass spectrometry (LC/MS/MS). Th e Heat acclimation index Δ SL Na and Δ Na calculated were the diff erences were calculated by subtracting the urinary sodium of before sealing from those of aft er sealing and before work shift from those of aft er work shift , respectively. Navy boiler tenders with better heat acclimation had a higher decreased of U-8OHdG than Navy boiler tenders with worse heat acclimation. Use of biologic markers may more accurately refl ect total dose of exposure in populations. We used U-8OHdG as a biomarker to refl ect the eff ect of health burden of DNA damage among naval personnel under environmental heat stress. U-8OHdG can be used as eff ective biomarker heat acclimatization in occupational medicine.