Hedera nepalensis: A novel source of natural cancer chemopreventive and anticancerous compounds

4^th World Congress on Cancer Science & Therapy

October 20-22, 2014 DoubleTree by Hilton Hotel Chicago-North Shore Conference Center, USA

Laila Jafri, Samreen Saleem, Ihsan-ul-Haq, Tamara P Kondrytuk, John M Pezzuto and Bushra Mirza

Accepted Abstracts: J Cancer Sci Ther

Abstract :

Traditionally medicinal plants are used for prevention as well as for the treatment of several diseases. Considering the medicinal importance of Hedera nepalensis based on traditional information, the present study was undertaken to analyze in vitro cancer chemopreventive and anti-proliferative sulforhodamine B (SRB) properties of the plant and a compound isolated from it i.e. lupeol. The in vitro cancer chemopreventive testing was performed using quinone reductase (QR) induction assay, inhibition of TNF-α activated nuclear factor kappa-B (NFκB assay), Inhibition of lipopolysaccharide (LPS)-activated nitric oxide (NO) production in murine macrophage RAW 264.7 cells (iNOS assay) and aromatase inhibition assay by crude extract and its three fractions (n-hexane, ethyl acetate and aqueous). The anti-proliferative assay was evaluated on three types of cancer cell lines: MCF-7, MDA-MB-231 andHeLausing 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) reduction assay. The results of cancer chemopreventive assays show thatn-hexane and ethyl acetate fractions of tested plant were having promising cancer chemopreventive potential while lupeol has shown lowest IC50 (0.20 μM/ml) in NFκB assay.Crude extract and its fractions inhibited the growth of three cancer cell lines by more than 60% while IC50 value of lupeol varied from 2.32-10.2 μM/ml. HPLC-DAD based quantification of lupeol in different plant tissues demonstrated that only leaves of H. nepalensis contain lupeol (0.196 mg/100 mg dry weight). In the light of the data generated the novel properties of H. nepalensis harboring cancer chemopreventive and anti-proliferative agents have been discussed.

Biography :

Laila Jafri (age of 31 years) is PhD student of biochemistry Department, Quaid-i-Azam university Islamabad Pakistan. She has published 2 papers and 4 papers are submitted in well reputed journals.