High sensitivity and selectivity of human antibody attachment at the interstices between substrate-bound gold nanoparticle

International Conference and Exhibition on Biosensors & Bioelectronics

May 14-16, 2012 Embassy Suites Las Vegas, USA

Kuan-Jiuh Lin, Yi-Heui Hsieh and Chuen-Yuan Hsu

Accepted Abstracts: J Biosens Bioelectron

Abstract :

It is helpful for early diagnosis, staging, prognosis, and mediating the aggressiveness of viral infections and cancer by providing real-time information to doctor. In this report, a rapid and highly sensitive chip has been developed for detecting low concentration molecules or specific rare cells. Unlike time-consuming ELISA and flow cytometry, the advantages of the chip include low-cost, reusability, high sensitivity and easy preparation. This chip is based on durable gold nanostructures on transparent glasses with uniform spacing which having an average nano-particle size and inter-particle gap of 8 nm and 11 nm, respectively. According to results, the optical response of localized surface plasmon resonance (LSPR) is strongly dependent on the chemical/biological molecule binding location. The optical response of LSPR increases when binding molecules are immobilized at the inter-particle spacing.The anti-Human immunoglobulin G molecule was applied as target to detect. Chemical immobilization had been used as bridge between the Human immunoglobulin G molecules and chip. The experimental result indicates that the limit of detection for anti-Human immunoglobulin G molecules is 66.7 pM. In the next step, we will attempt to detect pathogens or specific rare cells (i.e. circulating tumor cells or cytotoxic T lymphocyte) with special functionalization due to the high sensitivity by using our Au-LSPR chips. Now mass-production of the Au-LSPR chips can be achieved according to our patented microwave-plasma methods. Moreover, a hand held equipment of UV absorption has been designed to replace the large apparatus. We hope the Palm-sized equipment for detecting specific molecules that can be applied to Point-of-care testing in the future.

Biography :

Kuan-Jiuh Lin received his Ph. D. in Chemistry in 1993 from National Taiwan University, Taiwan. He is a distinguished professor of Chemistry of National Chung Hsing University, Taiwan. He has authored over 100 published articles/books and over 30 patents.