hNSCs Migration towards BTSCs in vitro and in vivo

4^th World Congress on Cancer Science & Therapy

October 20-22, 2014 DoubleTree by Hilton Hotel Chicago-North Shore Conference Center, USA

Liu Shuang, Yin Feng, Wang Shuwei, Guo Xinru and Zhang Jianning

Accepted Abstracts: J Cancer Sci Ther

Abstract :

Objective: To clarify the capacity of selective migration of human neural stem cells (hNSCs) to brain tumor stem cells (BTSCs) derived from glioblastoma, we studied the tropism of hNSC to BTSCs in vitro and in vivo. Methods: We cultured BTSCs from GBM tissues by neurosphere culture method, then, investigated the tropism ability of hNSCs to BTSCs using in vitro transwell chamber cell migration assay and in vivo tumor tropism studies. Results: The BTSCs could be obtained from human GBM tissues, and formed neuroshpheres after cultured 7d in vitro, and were CD133- positive and nestin-positive. Theses BTSCs could regenerate a GBM tumor in vivo when implanted intraventricularly. Cell migration assay showed that BTSCs have the capacity to actively attract hNSCs. The fate of hNSCs in vivo can be visualized under confocal fluorescence microscope. hNSCs exhibit extensive tropism toward the site of the tumor and infiltrate tumor foci. Conclusions: BTSCs could be derived rapidly from GBM tissues by neurosphere method. The normal hNSCs possess the capacity to migrate toward BTSCs in vitro and in vivo. The tropism of hNSCs towards brain tumors may provide an additional tool for the treatment of brain cancer.