Hormonal manipulation induces differentiation of pancreatic progenitor cells into insulin-secreting islet β-cells

Joint Meeting on 4^th World Congress on Human Genetics & Genetic Diseases and 3^rd International Conference on Molecular Medicine & Diagnostics

April 19-20, 2018 Dubai, UAE

Wen Xiong

Sichuan Provincial People′s Hospital, China

Scientific Tracks Abstracts: J Mol Genet Med

Abstract :

Diabetes is a kind of metabolic disease, which causes considerable morbidity in the world. Although pancreas transplantation and islet transplantation has prominent future, we still confront the main difficulty of organ shortage. Thus, a primary and fundamental effective therapy for diabetes is to develop ways to increase beta cell numbers. Here, we reported that under the stimuli of hormones, pancreatic duct epithelial cells, also known as pancreatic progenitor cells, could be differenced into insulin-secreting islet �²-cells. In this study, we collected pregnant rat serum and added to the culturing medium of isolated rat pancreatic duct epithelial cells. After seven days of culturing, the pancreatic progenitor cells will be aggregated. Then we compared the gender difference of the pancreatic progenitor cells and also the dosage of pregnant serum on the efficiency of differentiation. All cells treated with pregnant serum experienced expansion (A-D), aggregation (E-H) and islet-like cells formation (I-L) stages. Higher concentration of pregnant serum treated cells (L, J) generates more islet-like cells compared to those of lower ones (I, K). Pancreatic duct epithelial cells isolated from female rats formed larger islet-like sphere compared to those of male ones. However, pancreatic duct epithelial cells cultured with FBS could not form islet-like cells (N, low concentration of FBS control; O, high concentration of FBS control). Then the differenced islet-like cells were determined by dithizone staining (P) and aggregated pancreatic duct cells were determined by insulin staining (M). Judged from these two pictures, no matter the aggregated cells or the sphere-shaped ones are capable of secreting insulin. In conclusion, the pancreatic progenitor cells could be differenced to insulin-secreting islet �²-cells by the pregnant serum, which indicates the therapeutic potential of hormone therapy in preventing and/or treating diabetes.

Biography :

Wen Xiong has been engaged in ultrasonic diagnosis for 12 years; mastered the abdomen and superficial tissue disease; specialize in fetal abnormalities of prenatal ultrasound diagnosis and the ultrasonic diagnosis of children abdominal and superficial tissue diseases.
Email:1351233@qq.com