Identification and isolation of Iranian scorpion venom peptides of Odonthobuthus doriae using chromatographic techniques

16^th World Congress on CHEMISTRY AND MEDICINAL CHEMISTRY

March 27-28, 2023 | London, UK

Adel Abdollahnia

Shahid Beheshti University, Iran

Scientific Tracks Abstracts: J Med Chem

Abstract :

Scorpion venom is a complex mixture of salts, neurotoxins, peptides, and proteins. The application of scorpion venom for medicinal purposes dates back to ancient times. Most of the scorpion venom components show potential therapeutic activities against a variety of diseases such as Cancer, and bacterial, viral, and fungal infections. Anti-cancer properties are the main effect of scorpion venom and return to its inhibitory activities of the metalloproteinase matrix leading to motility reduction and invasion in tumor cells. In this study, the growth of Odonthobuthus doriae scorpion, identified for the first time in Fars province, was optimized in the scorpion production farm in terms of age, type of nutrition and breeding environment, and poisoning method with the lowest amount of lipids in the venom. For isolation and identification of Odonthobuthus doriae scorpion peptides, High-Performance Liquid Chromatography (RP-HPLC), Ultra Performance Liquid Chromatography (UPLC), Liquid Chromatography- Mass Spectrometry (LC-MS) apparatus accompany Sodium dodecyl sulfate-polyacrylamide gel (SDS-PAGE) test in One-dimensional (1D), two-dimensional (2D) was used. Recent Publications 1. Abdel-Mottaleb Y, Clynen E, Jalali A, Bosmans F, Vatanpour H, Schoofs L, Tytgat J. The first potassium channel toxin from the venom of the Iranian scorpion Odonthobuthus doriae. FEBS letters. 2006 Nov 13;580(26):6254-8. 2. Quintero-Hernández V, Jiménez-Vargas JM, Gurrola GB, Valdivia HH, Possani L. Scorpion venom components that affect ionchannels function. Toxicon. 2013 Dec 15;76:328-42.

Biography :

Adel Abdollahnia is a Ph.D. candidate in “Medicinal Chemistry” at Medicinal Plants and Drugs Research Institute (MPDRI) at Shahid Beheshti University (The best-ranking institute in Mass Spectrometry in Iran), Tehran, Iran.