Identification of cfr positive Staphylococcal aureus isolate from an ICU case

Global Cancer Conference & Medicare Summit

September 15-17, 2014 Hyderabad International Convention Centre, India

Guru Prasad Manderwad, Vidudala V T S Prasad, Lunavath Ravi Kumar and Ashok Kumar Reddy

Posters: J Cancer Sci Ther

Abstract :

Introduction: A Multidrug resistant bacterium poses a formidable challenge in effectively treating infections and is significant factor infection related mortality. The cfr (Chloramphenicol-flrofenicol resistance) gene encodes methyltransferase which methylates 23S rRNA at A2503, conferring resistance to various classes of antibiotics, such as oxazolidinone, phenicols, lincosamides, pleuromutilins streptogramin A. We report, presence of cfr related drug resistance in methicillin resistant Staphylococcus aureus isolated from septisemic patient. Material and Methods: A total of 59 bacterial isolates obtained from ICU (20) and ocular (39) cases from hospitals across the Hyderabad region were screened for the presence of cfr gene, using PCR with specific primers, and a positive control (The plasmid containing cfr gene was a gift from Dr. Gopegui ER, Hospital Universitari Son Espases, Palma de Mallorca, Spain). Result: Our investigations, revealed presence of cfr positive Staphylococcal isolate obtained from an ICU case. The presence of the multidrug cfr gene is a rare in the humans. The bacterial isolate was also found to have higher MIC to linezolid. The presence of the gene is being validated by sequencing. Conclusion: The present study demonstrates the presence of a cfr positive Staphylococcus aures. The particular isolate had a higher MIC for linezolid. Acknowledgement: The study was supported by Fast Track Scheme for Young Scientist (to Dr. GPM) by SERB under DST SB/ FT/LS-321/2012).