Identification of genes involved in bio film formation of mycobacteria in relation to susceptibility of antimicrobial agents

Joint Event on 9^th International Conference on Emerging Infectious Diseases & 6^th World Congress on Control and Prevention of HIV/AIDS, STDs & STIs

August 27-28, 2018 | Zurich, Switzerland

Virendra Kumar

National Jalma Institute for Leprosy, India

Posters & Accepted Abstracts: J AIDS Clin Res

Abstract :

Bacterial biofilms are often associated with infections especially with medical implants such as catheters and other medical devices. Biofilms are formed to protect the bacteria from host defences, antibiotics and from harsh environmental conditions. We had taken four mycobacterial species (M. smegmatis, M. fortuitum, M. avium and M. tuberculosis) for study of mycobacterial biofilm. The isolates were subcultured and characterized biochemically and molecularly. The large quantity of biofilm was produced by M. smegmatis at temperature 37ºC and 42ºC as compared to 30ºC. M. fortuitum developed more amount of biofilm at 30ºC as compared to 37ºC and 42ºC. M. avium developed strong amount of biofilm at 30ºC and 42ºC as compared to 37ºC. M. tuberculosis (H37Rv) developed strong biofilm at 37ºC and no biofilm at 30ºC and 42ºC in Middlebrook 7H9 broth (MB7H9) media and Sautonâ??s media. The selected non tuberculosis mycobacteria and H37Rv developed strong biofilm in the presence of OADC enrichment in MB7H9 as well as Sautonâ??s medium. Antibiotic susceptibility of biofilms at ultrastructural level was also studied in fast growing clinical isolates of M. smegmatis in presence of streptomycin, isoniazid rifampicin, ethambutol and pyrazinamide. Isoniazid showed strong inhibited biofilm in fast grower and sensitive isolates. However, pyrazinamide and isoniazid inhibited biofilm of M. tuberculosis (H37Rv) and in MDR isolates ethionamide and moxifloxacin inhibited biofilm in slow grower and fast grower mycobacteria. However, many mycobacterial species are known to form biofilms, little is known about either the genetic requirements, patterns of gene expression. In micro array hybridization we have found that six genes were expressed in M. avium. In M. tuberculosis MDR isolates seven genes were expressed and two genes Rv0359 and Rv3526 were homologues as earlier reported in P. areuginosa and M. avium which might be responsible for biofilm formation.

Biography :

E-mail: vksjalma@gmail.com