Identification of new potent inhibitor of aldose reductase from Ocimum basilicum

5^th International Conference on Organic and Inorganic Chemistry

July 12-13, 2018 | Paris, France

Huma Aslam Bhatti

University of Karachi, Pakistan

Posters & Accepted Abstracts: J Chemical Sci

Abstract :

Recent efforts are to develop cure for chronic diabetic complications results in the discovery of potent inhibitors against aldose reductase (ALR2, EC 1.1.1.21) whose role in diabetes is well-evident. In the present work, two new natural products were isolated from the aerial part of Ocimum basilicum; 7-(3-hydroxypropyl)-3-methyl-8-�²-O-D-glucoside-2H-chromen-2-one (1) and E-4-(6'- hydroxyhex-3'-en-1-yl) phenyl propionate (2) and confirmed their structures with different spectroscopic techniques including NMR spectroscopy etc. The isolated compounds (1, 2) were evaluated for in vitro inhibitory activity against aldose reductase (ALR2) and aldehyde reductase (ALR1). The natural product (1) showed better inhibitory activity for ALR2 with IC50 value of 2.095�±0.77 �¼M compare to standard sorbinil (IC50=3.14�±0.02�¼M). Moreover, the compound (1) also showed multifolds higher activity (IC50=0.783�±0.07�¼M) against ALR1 as compared to standard valproic acid (IC50=57.4�±0.89�¼M). However, the natural product (2) showed slightly lower activity for ALR2 (IC50=4.324�±1.25�¼M). Moreover, the molecular docking studies of the potent inhibitors were also performed to identify the putative binding modes within the active site of aldose/aldehyde reductases.