Jamal Hussen, Turke Shawaf and Ahmed M Alluwaimi
King Faisal University, Saudi Arabia
Posters & Accepted Abstracts: J Vet Sci Technol
Some species like cattle, sheep and poultry are known to have a high percentage of �³�´ T cells in their peripheral blood and they are therefore, referred to as �³�´ T cell high species bovine �³�´ T cells, which play a role in immune defense on epithelial and mucosal surfaces, may comprise up to 60% of blood mononuclear cells in young calves and decrease as the animals age. Whether camels belong to this group of species with high �³�´ T cell proportion in blood, it is unknown. In the current study, flow cytometry was used to evaluate the effect of age on the percentage and immunophenotype of �³�´ T cells in the peripheral blood of dromedary camels. Dromedary camels were grouped according to their age as young (1-5 years old) and old (611 years old) camels. Separated camel PBMC were incubated with monoclonal antibodies cross-reactive with camel �³�´ T cell antigen (WC1) and the cell surface molecules, CD62L, CD11a, CD11b and CD18 labelled cells were analyzed by flow cytometry. The percentages of WC1-positive �³�´ T cells were higher in young camels in comparison to older camels. Although the expression level of CD11a, CD11b and CD18 on WC1-positive cells did not differ between young and old animals, old camels showed reduced expression level of CD62L in comparison to young animals. Our results showed that camels belong to the �³�´ T cell high species. In addition, the higher expression levels of CD62L on WC1-positive of older camels may indicate a special age-dependent role for this cell adhesion molecule in the �³�´ cell migration in camels.
Recent Publications
1. Chen C, H Hsu, E Hudgens, J C Telfer and C L Baldwin (2014) Signal transduction by different forms of the gammadelta T cell-specific pattern recognition receptor WC1. Journal of Immunology 193(1):379-390.
2. Guzman E, J Hope, G Taylor, A L Smith, C Cubillos-Zapata and B Charleston (2014) Bovine gammadelta T cells are a major regulatory T cell subset. Journal of Immunology 193(1):208-222.
3. Holm D, D R Fink, J Gronlund, S Hansen and U Holmskov (2009) Cloning and characterization of SCART1, a novel scavenger receptor cysteine-rich type I transmembrane molecule. Molecular Immunology 46(8-9):1663-1672.
4. Graff J C and M A Jutila (2007) Differential regulation of CD11b on gammadelta T cells and monocytes in response to unripe apple polyphenols. Journal of Leukocyte Biology 82(3):603-607.
5. Li C Q, Y J Xu, S X Chen, W Ni, X N Zhong, D L Yang, X S Liu and Z X Zhang (2004) The isolation and expansion of gamma delta T cells in peripheral blood and bronchoalveolar lavage fluid. Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi 20(3):337-339.
Jamal Hussen collected his experience in Veterinary Immunology after many years of research and teaching at different international veterinary institutions. In his current research activity, he focuses on the characterization of the cellular immune system in dromedary camel.
E-mail: jhussen@kfu.edu.sa
Veterinary Science & Technology received 4472 citations as per Google Scholar report