Impact of herbal supplementation in-vivo on oxidative stress markers and semen quality in subfertile buffalo (Bubalus bubalis) bulls

9^th International Conference and Exhibition on Chinese Medicine Ayurveda & Acupuncture

March 12-13, 2018 | Barcelona, Spain

Shiv Kumar, A Kumar, M Honparkhe, A K Singh, P Malhotra, V K Gandotra and P Singh

Guru Angad Dev Veterinary and Animal Sciences University, India

Posters & Accepted Abstracts: Altern Integr Med

Abstract :

Study was conducted to assess the combined effects of herbs on semen quality and oxidative stress markers in sub-fertile buffalo bulls (Bubalus bubalis). A total of 144 semen ejaculates (16 ejaculates/bull) were collected from 3 sub-fertile buffalo bulls maintained at bull station, Guru Angad Dev Veterinary and Animal Sciences University, Ludhiana, India (Latitude/ Longitude, 30.55�°N, 75.54�°E) following oral supplementation of Panax ginseng roots, shilajit, Withania somnifera roots, Tribulus terrestris fruits, Turnera diffusa leaves, Ptychopetalum olacoides bark each @400 mg/100 kg body weight and Pausinystalia yohimbe bark @300 mg/100 kg body weight of bulls for 60 days. Semen was evaluated for quality and oxidative stress markers (lipid peroxidation, superoxide dismutase and glutathione peroxidase) during pre-supplementation, supplementation and post-supplementation phases (each phase of 60 days). Data were analyzed by general linear univariate-model with bull as random factor using SPSS software. Herbal supplementation significantly (P<0.05) improved mass motility, individual motility, rapid progressive motility, curvilinear velocity, sperm concentration, viability and active mitochondria. Total sperm abnormalities and amplitude of lateral head displacement were significantly (P<0.05) reduced during supplementation and post-supplementation phases. The level of malondialdehyde was significantly (P<0.05) declined during supplementation and post-supplementation phases. The activity of superoxide dismutase significantly (P<0.05) increased during supplementation and post-supplementation phases, whereas the activity of glutathione peroxidase was significantly (P<0.05) increased only during the supplementation phase. It could be concluded that feeding of herbs in combination improved the semen quality and antioxidants activity in sub-fertile buffalo bulls. kamthaneshivkumar@gmail.com