Impact of L-arginine (a nitric oxide synthesis precursor) and N-É·-nitro-L-arginine-methyl-ester (L-NAME) (a non-selective nitric oxide synthase inhibitor) chronic, subchronic and acute toxicity-induced lesions on asci

3^rd International Veterinary Congress

August 18-20, 2016 London, UK

Marina Tisljar, Z Grabarevic, Branka Artukovic, P Dzaja, Jadranka Forsek and Renata Baric Rafaj

Croatian Veterinary Institute, Croatia
University of Zagreb, Croatia

Posters & Accepted Abstracts: J Vet Sci Technol

Abstract :

Experiment A. Chronic toxicity. 140 one day-old male broiler chicks divided in four groups. Every other day: intraperitoneal (ip) L-NAME (LN, 10 mg/kg BW); L-arginine (LA, 100 mg/kg BW), L-arginine and a L-NAME combination (100 and 10 mg/kg BW, respectively), and physiological saline (0.90% w/v; 0.5 mL/kg BW). Seven birds were euthanized weekly during their first five weeks of life. Experiment B. Subchronic toxicity. Equal doses of the same substances: ip every eight hours into 38 day-old birds over 36 hours. Experiment C. Acute toxicity. Thirty day-old birds: one ip dose of various concentrations of L-NAME (50, 100 and 150 mg/kg BW); L-arginine (100 mg/kg BW) and saline (0.5 ml/kg BW). All the birds were euthanized after six hours. The end of experiment A: the gross finding of ascites-pulmonary hypertension syndrome (PHS) confirmed vasoconstrictory effect of L-NAME in five birds (LN). Towards the end of experiment A; in experiment C: histopathological findings (myocardial/ pulmonary oedema/LN/; congestion/haemorrhages/LA/) the most prominent (higher LN-doses/100 and 150 mg/kg BW/). Irreversible myocardiolysis and hepatocellulolysis: confirmed in all three experiments (LN). Focal myocardial degeneration solely: in the L-NAME/L-arginine simultaneously treated group. Haematological and blood chemistry values; stress index value; and relative organ weights agreed with well-known literature data on PHS. Experiments A and C. The severity of LN-provoked hypoxic changes and plentiful haemorrhages (LA) - dose and time dependent. Mild changes in L-NAME/L-arginine group confirmed a protective role of LA.

Biography :

Email: marina.tisljar@gmail.com