In vivo and in vitro analysis of cancer by Raman-IR-SNOM-AFM imaging and femtosecond spectroscopy - From single cells to humans

European Chemistry Congress

June 16-18, 2016 Rome, Italy

Halina Abramczyk

Lodz University of Technology, Poland

Posters & Accepted Abstracts: Chem Sci J

Abstract :

This contribution will explore cutting edge molecular (Raman, IR, fluorescence, SNOM, AFM, TERS, femtosecond spectroscopy) mapping and time resolved dynamics of cellular structures of cancers, localization of drugs and nanoparticles in cells and tissues. The multidisciplinary nature of the studies span the a diverse range of biological, chemical, and physical sciences related to cancer biology. This contribution will provide insight regarding the new molecular mapping and their ability to monitor biochemistry of biomolecules in the cells and tissues, distribution of drugs, and nanomaterials as they interact with cells and tissues. The main focus will be on the presentation of integrated picture of cancer by near field microscopy SNOM, AFM and hyperspectral Raman imaging to look inside human breast ducts. We will demonstrate how this approach gives important answer about location and distribution of biochemical components in human cells and tissue during cancer development. The lecture shows new look inside human breast duct using Raman imaging, an emerging technology of molecular imaging, that may bring revolution in understanding of cancer biology. Our contribution is a first report in the literature demonstrating such a detailed analysis of normal and cancerous ducts in human breast tissue. The main advantage of Raman imaging is that it gives spatial information about various chemical constituents in defined cellular organelles in contrast to conventional methods (LC/MS, NMR, HPLC) that rely on bulk or fractionated analyses of extracted components. abramczy@mitr.p.lodz.pl