Inclusion complexes of propiconazole nitrate with substituted β-cyclodextrins II: In vitro assessment of antifungal properties

International Conference and Exhibition on Materials Chemistry

March 31-April 01, 2016 Valencia, Spain

Irina Rosca, Bogdan Minea, DragoÃ?Â? Peptanariu, Narcisa Marangoci, Adrian Fifere1 and Mihai Mares

Petru Poni Institute of Macromolecular Chemistry, Romania
Ion Ionescu de la Brad University, Romania

Posters & Accepted Abstracts: J Material Sci Eng

Abstract :

Candida albicans infections are an important health issue fuelled, paradoxically, by the advancements in medical care. The prophylactic administration of antifungals generates antifungal resistance and this underlines the need for new antifungal agents. Inclusion complexes of protonated propiconazole nitrate (PCZH-NO3) with three substituted Cyclodextrin (CD) derivatives, namely sulfobutylether �² CD (SBE7 �² CD), sulfated �² CD (�² CD SNa) and monochlorotriazinyl �² CD (MCT �² CD) were investigated as new antifungal systems. The antifungal activity of the inclusion complexes was assessed on 20 Candida spp. clinical isolates. The in vitro susceptibility testing was performed following the EUCAST EDef 7.2 guideline. To assess the cytotoxicity, the CellTiter 96�®aqueous one solution cell proliferation assay was performed on Normal Human Dermal Fibroblasts (NHDF). All complexes showed antifungal activity at low concentrations. The IC50 values were two to three orders of magnitude higher than the concentrations required for antifungal activity. The 95% CIs indicate a significantly higher cytotoxicity for the complex with the parental �² CD compared to those with the other three CD derivatives. The much lower concentrations required for the antifungal effect, compared to the IC50 cytotoxicity values, prove a high selectivity of the active compound for the fungal cells. The lack of significant differences in the antifungal susceptibility tests and the differences in cytotoxicity between the �²-CD complex and the other three suggest that the type of cyclodextrin may be more important for the interaction with the human organism than it is for the actual antifungal activity.

Biography :

Irina RoÃ?Â?ca has completed her PhD in Biology at Faculty of Biology from Al I Cuza University, Iasi, Romania and is a Scientific Reseacher at Centre of Advanced Research in Bionanoconjugates and Biopolymers from Petru Poni Institute of Macromolecular Chemistry. She has published more than 10 papers, she was Principal Investigator in 1 project and worked in another 8 projects related to biotechnology, microbiology and ecology.

Email: rosca.irina@icmpp.ro