Inhibin B level influence on the DNA fragmentation degree of spermatozoids of males with infertility

International Conference and Exhibition on Molecular Medicine and Diagnostics

August 24-26, 2015 London, UK

Lazzat Tuleyeva*, Mirzakarim Alchinbaev, Dinara Makazhanova and Marat Makazhanov

Posters-Accepted Abstracts: J Mol Genet Med

Abstract :

Background/Aim: Determine the influence of the level of inhibin B on the degree of DNA fragmentation of sperm.
Materials and Methods: Age of researched patients (50 male) with a diagnose of infertility varied 25 to 35 years, and in average
it was 31.4±1.8 years, and in control group it was 32.30± 1.11 years (p > 0.05). For carrying out analysis of spermatozoids DNA
fragmentation there was used a SCD method (sperm chromatin dispersion, Halosperm, Spain). The analysis was carried out with the
help of fluorescent microscope Axioscop 40. Spermatozoids with fragmented DNA should not exceed 20.0 % at standard conditions. In
blood plasma an inhibin B level was determined by the Elisa method on a map-board analyzer Stat FAX2100 (Awareness Technology
Inc).
Research Results: Healthy males’ level of inhibin B showed 341 pg/mL on average, while males with infertility had decreased level of
inhibin B up to 128 pg/m, that is 37.5 % less than healthy males. Correlation connection between spermatozoids DNA fragmentation
indexes and inhibin B have been registered against inhibin B level decrease tendency at males of a main and control groups aged 36
to 45. Inhibin B level in a blood plasma was lower at 21 males of main group, three of which was diagnosed asthenoazoospermia, 6–
oligospermia, and 12– asthenozoospermia. The following chart shows dependence of spermatozoids DNA fragmentation on inhibin
B level in a blood. Patients with pathozoospermia containing inhibin B within standard index (147 – 364 pg/mL), had 23% to 27
% of spermatozoids DNA fragmenatation level. With decreasing of inhibin B level up to 128 pg/mL proportionally the degree of
spermatozoids DNA fragmentation in an ejaculate has increased from 31 % to 40%.
Conclusion: Inhibin B has much higher importance and is more essential marker in male fertility disorder, low concentration of
inhibin B points at changes in spermatogenesis.

Biography :

Lazza Tuleyeva - MPH. Senior Researcher in theLaboratory of molecular diagnostics, Scientific Center of Urology named BU Jarbussynov, Almaty, Kazakhstan. She has
published more than 30 papers in reputed journals.