Inhibitory effect of amniotic membrane proteins in HSP60 gene expression in HepG-2 Hepatocarcinoma cell line

4^th World Congress on Cancer Science & Therapy

October 20-22, 2014 DoubleTree by Hilton Hotel Chicago-North Shore Conference Center, USA

Alireza Nourazarian, Seyed Manuchehr Nourazarian and Majid Tozihi

Accepted Abstracts: J Cancer Sci Ther

Abstract :

Introduction: In recent years HSP90 has been studied intensively as a therapeutic target. Hsp90 is an abundant protein in mammalian cells which is responsible for the consolidation of a many of cellular pathways and processes. Human amniotic membrane (hAM) has recently been considered as an upcoming anti-cancer therapy. The aim of this study was investigation the effect of hAMP on the expression levels of HSP60 gene in HepG-2 Hepatocarcinoma cell line. Material and methods: hAMPE were mechanically obtained, after quantification of proteins in hAMPE, their effect on the expression levels of HSP60 gene in HepG-2 Hepatocarcinoma cell line was evaluated. For each experiment, cells were incubated with 1 lg/lL of hAMPE. The extraction of total cellular RNA, cDNA synthesis and Real Time PCR was done for all of samples. We used western blot analysis to elucidate the effect of hAMP on the level of Hsp60 protein expression. Statistical analysis was performed with SPSS software. Statistically comparisons were between the control group and treatment. Results: The results showed have an effective role in decrease of Hsp-60 expression. Our results demonstrated hAMPE had more than 68% inhibition effects on the Hsp-60 expression. Western blot results show Hsp60 expression had been decreased about 56% (P<0.05). Conclusions: According to results, it would appear that inhibition of HSP60 by hAMPE can provide therapeutic opportunities in the field of cancer treatment. We suggest that further studies should attempt in vivo experiments on laboratory animals to investigate the efficacy of these proteins in biological environments.