Intravenous injection of lipopolysaccharide increases expression of inflammatory cytokines in murine brain: Possible the role of norepinephrine

International Congress on Neuroimmunology and Therapeutics

DoubleTree by Hilton Hotel San Francisco Airport, San Francisco, CA, USA

Zahra Abrehdari-Tafreshi1, Parvaneh Allahdini1, Majid Pirestani2 and Elham Safarpour1

Posters-Accepted Abstracts: J Neurol Disord

Abstract :

Evidence suggests that noradrenaline has a tonic anti-inflammatory activity in the central nervous system (CNS) owing to its ability to suppress microglial and astrocytic activation, as well as inhibiting production of inflammatory mediators. Therefore it is proposed that noradrenaline may play an endogenous neuroprotective role in CNS disorders where inflammatory events contribute to pathology. Here we survey anti-inflammatory effects of norepinephrine on tumor necrosis factor-α (TNF-α) and interleukin-6 (IL-6) gene expression following a systemic challenge with bacterial lipopolysaccharide (LPS) in the mouse. In the present study twenty one male NMRI mice (25 ± 5 g) used and divided into three groups (n = 7). They were injected through the mouse tail vein with saline, 100 nM norepinephrine 30 min before the intravenous injection of LPS (5 mg/kg). Thus, inflammation induced by the intraperitoneal injection of LPS. The control animals received sterile saline in the first and second injections. Changes in the expression TNF-α and IL-6 genes studied in the mice brain by a semi quantitative RT-PCR method. The results of this study showed that norepinephrine makes alteration to the expression of IL-6 genes in brain 2 h before the injection of LPS. Norepinephrine markedly reduced the expression of IL-6 genes. The data suggest that norepinephrine protective effect in brain inflammation induced by LPS through inhibition IL-6. Overall, this study has yielded significant insights into the ability augmentation of noradrenaline strategies to limit neuroinflammation.