Isolation and structure elucidation harmine alkaloid from Rumex chalepensis growing in Iran

13^th International Conference on Alternative Medicine

June 24, 2022 | Webinar

Majid Halimi, Malihe Nasrabadi, Hamid Soorgi

Kosar University of Bojnord,Iran
Payame Noor University,Iran
North Khorasan University of Medical Sciences, Iran

Scientific Tracks Abstracts: Altern Integr Med

Abstract :

Alkaloids might be found in all plant parts but tended to accumulate in storage organs of plants. Alkaloids act as stimulators, inhibitors and growth terminators. Harmine has been traditionally used for medicinal preparations in the Middle East, Central Asia and South America. Harmine is widely distributed in nature, such as in various plants, marine creatures, insects, mammalians, human tissues and body fluids. Harmine have antimicrobial, antiplasmodial, antifungal, antioxidative, antitumor, antimutagenic, cytotoxic and hallucinogenic properties. Genus Rumex (Family: Polygonaceae) includes many edible plants which attracted the attention of many investigators because of their medicinal importance for the treatment of several diseases9.Some plants of this family contains alkaloids which have been used in traditional African system medicine. In our investigation, aerial parts of R. chalepensis were collected at the flowering stage from the Birjand, Iran, In Jun 2010 then alkaloids were exhaustively extracted corresponding with figure 2. The curde alkaloids combined with CHCl3 extract was subjected to MPLC and eluted with solvent system (CH2Cl2/MeOH)(10:1). A single MPLC separation step performed on the alkaloid extract gave 4 fraction, to finally Fractions with similar TLC behavior were combined to yield the following one major fraction (65 mg). The structure elucidation of this compound was established by spectroscopic methods, including EI-MS, IR, 1H, 13C-NMR experiments
Recent Publications :
1- Rowson, J. M. (1958) Alkaloids and plant taxonomt. Proceedings of the linnean society of London. 169: 212–216.
2- Waller, G. R. (1978). Alkaloid Biology and Metabolism in Plants Springer.
3- Nazrullaev, S. S., I. A. Bessonova and K. S. Akhmedkhodzhaeva (2001) Estrogenic activity as a function of chemical structure in Haplophyllum quinoline alkaloids. Chemistry of Natural Compounds. 37:551-555.
4- Egusa H, Doi M, Saeki M, Fukuyasu S, Akashi Y, Yokota Y (2011) The small molecule harmine regulates NFATc1 and Id2 expression in osteoclast progenitor cells. Bone. 49(2):264–274.
5- Réus GZ, Stringari RB, Gonçalves CL, Scaini G, Carvalho-Silva M, Jeremias GC (2012). Administration of harmine and imipramine alters creatine kinase and mitochondrial respiratory chain activities in the rat brain. Depress Res Treat. 98:73-97.
6- Yonezawa T, Hasegawa S, Asai M, Ninomiya T, Sasaki T, Cha BY, (2011). Harmine, a B-carboline alkaloid, inhibits osteoclast differentiation and bone resorption in vitro and in vivo. Eur J Pharmacol. 650(2-3):511–518.

Biography :

Majid Halimi studied Chemistry at the Ferdowsi University, Iran and graduated as MS in 2002. He then joined the research group of Prof. Vahedi and Lari at the PNU University Department of oOganic Chemistry, Academy of Sciences, Iran. He received his PhD degree in 2014 at the same institution.