Label enhanced SPR applied to the analysis of small molecules and biomolecules

6^th International Conference and Exhibition on Biosensors & Bioelectronics

September 22-23, 2016 Phoenix, USA

Anders Hanning and Lars Eng

Episentec, Sweden

Posters & Accepted Abstracts: J Biosens Bioelectron

Abstract :

Label Enhanced Surface Plasmon Resonance (LE-SPR) is a new concept which greatly improves the sensitivity and specificity of SPR analysis. LE-SPR can be used directly on standard Biacore� instruments and does not require any modification of the existing SPR instrument hardware. LE-SPR is based on labelling of one interactant with specialized dye labels combined with software based curve shape analysis of the entire SPR dip curve. An extremely sensitive and fully specific measure of the binding of dye labeled compound is obtained and plotted as an enhanced sensorgram or �epigram�. At first sight, the label enhanced concept seems not to fit into the label free paradigm. However, by selecting a competitive assay format where a label free analyte competes with a dye labeled analogue for binding to the surface, the interaction between the analyte and the surface is truly label free, while the SPR readout is label enhanced. By applying well established methods of competitive analysis, both the kinetics constants and the affinity of the label free analyte can be accurately determined.

Biography :

Email: anders.hanning@episentec.com