Ligands of TLR7/8 up-regulate IFN production in human trophoblast cell lines SWAN-71

3^rd International Conference on Diagnostic Microbiology and Infectious Diseases

September 24-25, 2018 | Montreal, Canada

Elaine Cristina Cardoso

University of Sao Paulo, Brazil

Posters & Accepted Abstracts: J Med Microb Diagn

Abstract :

Background: According to UNAIDS (Joint United Nations Programme on HIV/AIDS), women account for 50% of the people infected with HIV-1 worldwide. Nevertheless, HIV-1 mother-to-child transmission (MTCT) has been significantly reduced due to the use of antiretroviral therapies. The consequences of HIV-1 infection on the innate immune system of both mother and newborn, however, are not well understood. Evidence indicates that expression of Toll-like receptors (TLR) by trophoblast cells might regulate the differentiation and activation of immune cells, likely a key modulation for successful gestation and fetal protection. Methods: Herein, we analyzed the production and secretion of cytokines by trophoblast cells involved in an antiviral response. Trophoblast cell line (Swan-71) was activated with agonists for intracellular TLRs and infected with HIV-1. Results: The cytokines IL-6 and IL-8 were evaluated by cytometric bead array and TNF-α, IFN-α and IL-10 production by flow cytometry. Viral replication was also quantified. Conclusions: Our results showed a potential modulatory effect of CL097 (TLR7/8) and Gardimiquimod (TLR7), which may reflect an improved anti-viral response during pregnancy, associated with increased IFN-α production. These findings raise new possibilities in the search for better tools in the development of vaccine adjuvant as well as more efficient antiretroviral.

Biography :

E-mail: enialeccardoso@gmail.com