Malic acid boosted TCA cycle enhances survival of zebrafish to Vibrio alginolyticus infection

15^th International Conference on Metabolomics and Systems Biologys

August 29-30, 2019 | Vienna, Austria

Manjun Yang

Tibet Vocational Technical College, China
Sun Yat-sen University, China

Scientific Tracks Abstracts: Metabolomics (Los Angels)

Abstract :

Vibrio alginolyticus is a waterborne pathogen that infects a wide variety of hosts including fish and human, and the outbreak of this pathogen can cause a huge economic loss in aquaculture. Thus, enhancing hostâ??s capability to survive from V. alginolyticus infection is the key to fight infection and this remains still unexplored. In the present study, we established a V. alginolyticus-zebrafish interaction model by which we explored how zebrafish survived from V. alginolyticus infection. We used GC-MS based metabolomic approaches to characterize differential metabolomes between survival and dying zebrafish upon infection. Pattern recognition analysis identified the TCA cycle as the most impacted pathway. The metabolites in the TCA cycle were decreased in the dying host, whereas the metabolites were increased in the survival host. Furthermore, the enzymatic activities of the TCA cycle including pyruvate dehydrogenase (PDH), α-ketoglutaric dehydrogenase (KGDH) and succinate dehydrogenase (SDH) also supported this conclusion. Among the increased 3 metabolites in the TCA cycle, malic acid was the most crucial biomarker for fish survival. Indeed, exogenous malate promoted zebrafish survival in a dose-dependent manner. The corresponding activities of KGDH and SDH were also increased. These results indicate that the TCA cycle is a key pathway responsible for the survival or death in response to infection caused by V. alginolyticus, and highlight the way on development of metabolic modulation to control the infection.

References:

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Biography :

Manjun Yang is a lecturer of Tibet Vocational Technical College. He is pursuing his PhD from Sun Yat-sen University. His tutor is Prof. Xuanxian Peng who is a famous scientist of China. He has been committed to the research of bacterial antibiotic resistance. He is good at using metabolomic approaches based on GC-MS and UPLC/Q-TOF- MS platform to study drug resistance.

E-mail: manjunyang@126.com