Management of cystic fibrosis patient colonization using culture plate digital imaging technologies: Early growth detection of small colony variants and morphotype recognition by classical digital imaging and direct bact

Global Medical Microbiology Summit & Expo

November 28-29, 2016 San Francisco, USA

Geraldine Durand

bioMerieux, France

Posters & Accepted Abstracts: J Med Microb Diagn

Abstract :

Microbiological monitoring of pulmonary colonization is essential for Cystic Fibrosis (CF) patient management. However, many factors make microbiological culture analysis especially difficult for CF patients: Variants with atypical morphology and several morphotypes of the same species can coexist. Colonization with Pseudomonas aeruginosa and the conversion into a mucoid phenotype and into Small Colony Variants (SCVs) are critical steps in the course of the disease. Staphylococcus aureus SCVs must be recognized in order to start appropriate antibiotic therapy. The first goal of the study was to demonstrate that �?¢�?�?�?�?classical�?¢�?�?�? digital imaging allows for an earlier detection of the SCVs and also for recognition of the different colony morphotypes. However classical digital imaging still limits itself to what can currently be observed by the naked eye. The second goal was to show that hyperspectral imaging gives additional means to observe more characteristics linked to the construction of colonies and could lead to an optical species-specific fingerprint, enabling direct bacterial identification. Digital imaging technologies ensure early detection of any colonization with SCVs of P. aeruginosa and S. aureus and authorize the recognition and identification of morphotypes thus providing a powerful tool to track the different morphotypes that colonize CF lung. Therefore, digital imaging technologies convey a real medical added value for diagnosis and management of CF patients.

Biography :

Email: Geraldine.DURAND@biomerieux.com