Marburg cerebrospinal fluid (CSF) model reveals CSF constituents as new CSF biomarkers

7^th International Conference and Expo on Molecular & Cancer Biomarkers

September 15-16, 2016 Berlin, Germany

Tilmann O Kleine

Universit�¤tsklinikum Giessen & Marburg, Institut Laboratoriumsmedizin & Pathobiochemie, Germany

Posters & Accepted Abstracts: J Mol Biomark Diagn

Abstract :

In healthy humans CSF (total: â��150 ml) flows from ventricles V1-V4 into subarachnoid/spinal spaces, where CSF is absorbed. CSF cellular and protein constituents are controlled by blood-CSF barrier (bCSFb) in 4 choroid plexus (main CSF production) and blood-brain barrier (bbb) in brain capillaries, sealed with tight-tight junctions. Cellular constituents are leukocytes (mainly lymphocytes): 0-1/�¼l in ventricle V-CSF, 1-3/�¼l in cistern C-CSF, 1-5/�¼l in lumbar L-CSF; representing 2 cellular biomarkers: `V/C-CSF leukocytes` evaluate penetration of blood leukocytes pressed through circum-ventricular organs (without bbb) into V-CSF by blood pressure; - `L-CSF lymphocytes` uncover reflux rate of lymph lymphocytes when lumbar CSF drains out into thoracic duct. L-CSF leukocytes are sum of C-CSF blood leukocytes + L-CSF lymph leukocytes. CSF protein levels are dependent on their molecular weight: albumin >IgG >IgA >IgM and reveal filtration of blood proteins into V-CSF through bCSFb less-tight junctions and basal membrane (no barrier with plexus capillaries/venules). Biomarkers `CSF proteins` reveal a) penetration capacity of blood proteins through bCSFb into V CSF, b) flow rate of V-CSF into L-CSF, c) concentration of CSF draining along cranial and spinal nerves: small proteins (albumin) drain out easier than large ones (IgM); thus summarizing the CSF sink effect. Summary: The new biomarkers renew CSF patho-biochemistry, thus improving human CSF diagnostics, revealed with Marburg CSF Model.

Biography :

Email: kleine@uni-marburg.de