Metabolomic profile of low copy-number carriers at the salivary α-amylase gene suggests a metabolic shift towards lipid-based energy production

7^th International Conference and Expo on Metabolomics

November 14-16, 2016 Orlando, Florida, USA

Abdelilah Arredouani

Hamad Ben Khalifa University, Qatar

Scientific Tracks Abstracts: Metabolomics

Abstract :

Low serum salivary amylase levels have been associated with a range of metabolic abnormalities, including obesity and insulin resistance. We recently suggested that low copy-number at the AMY1 gene, associated with lower enzyme levels, also increases susceptibility to obesity. To advance our understanding of the effect of AMY1 copy-number variation on metabolism, we compared the metabolomics signatures of high and low copy-number carriers. We analyzed, using mass spectrometry and NMR, the sera of healthy normal-weight women carrying either low (LA: â�?¤4 copies; n=50) or high (HA: â�?¥8 copies; n=50) AMY1 copies. Best fitting multivariate models (empirical P<1x10-3) of MS and NMR data were concordant in showing differences in lipid metabolism between the two groups. In particular, LA carriers showed lower levels of long- and medium-chain fatty acids, and higher levels of di-carboxylic fatty acids and 2-hydroxybutyrate (known marker of glucose mal-absorption). Taken together, these observations suggest increased metabolic reliance on fatty acids in LA carriers through �?²- and Ï�?-oxidation and reduced cellular glucose uptake with consequent diversion of acetyl-CoA into ketogenesis. Our observations are in line with previously-reported delayed glucose uptake in LA carriers after starch consumption. Further functional studies are needed to extrapolate from our findings to implications for biochemical pathways.

Biography :

Abdelilah Arredouani has joined QBRI in March 2012 as a Scientist. He comes from Weill Cornell Medical College in Qatar where he served since March 2010 as a Senior Postdoctoral Research Associate in the Department of Physiology and Biophysics, where he studied the trafficking of Orai1, the calcium channel that mediates the store-operated calcium influx in non-excitable cells. He holds a special Master’s in Molecular Biology and Biotechnology Management and a PhD in Cell Physiology from Belgium. During his PhD, he studied the role of the endoplasmic reticulum calcium stores in glucose-induced insulin secretion from the pancreatic beta cell in the context of type-2 diabetes.

Email: aarredouani@qf.org.qa