Microbiological examination of fish pond biofilms and fish pond water from selected fish ponds, Okigwe, Imo state, Nigeria

Joint Conference on 6^th Annual Conference on Microbiology & Annual Conference on Microbes and Beneficial Microbes

October 16-17, 2017 Baltimore, USA

Chukwu Vince Anyim

Federal University, Nigeria

Posters & Accepted Abstracts: J Med Microb Diagn

Abstract :

The microbiological examination of fish pond biofilms and fish pond water in Okigwe town, Imo State, Nigeria was carried out in this study using standard procedures. The bacterial species isolated were Escherichia coli, Enterobacter, Staphylococcus, Streptococcus, and Salmonella. Others were Pseudomonas species, Proteus species, Shigella, Bacillus, Citrobacter and Klebsiella species. The fungi isolated include Aspergillus species, Candida, Penicillium, Fusarium and Mucor species. Bacillus spp. and Mucor spp were the most frequently distributed (100%), followed by Staphylococcus aureus, E. coli, Pseudomonas spp., Proteus spp., Shigella spp and Candida species (80%). Salmonella spp., Klebsiella spp., Citrobacter spp, Enterobacter spp, Fusarium spp and Penicillium spp had the same rate of 60%, respectively while the least occurrence was seen in Streptococcus spp. and Aspergillus species with the rate of 40%, respectively. The highest total bacterial count (7.2�±0.0��107 cfu/ml) of the biofilm samples was observed in sample C while sample D had the least count of 5.8�±0.5��1010 cfu/ml. Sample C had the highest total coliform count (6.2�±0.0��104 cfu/ml) while sample B produced the least count of 4.8�±0.1��103 cfu/ml. The highest total coliform fecal count of 4.8�±0.1��105 cfu/ml was observed in sample E, while the least count of 3.6�±1.5��102 cfu/ml was seen in sample B. Sample E had the highest total fungal count of 5.2�±0.0��102cfu/ ml while the least count was seen in sample D (4.0�±0.2��102 cfu/ml). The total viable counts of the fish pond water samples ranged from 8.2�±0.8��108 cfu/ml (sample A) to 5.0�±0.0��107 cfu/ml (sample C) while the total coliform counts ranged from 6.0�±0.0��103 cfu/ml (sample A) to 4.2�±0.2��101 cfu/ml (sample E). The highest total fecal count of 5.5�±0.2��103 cfu/ml was observed in sample A, while the least count was seen in the sample E 3.0�±0.1��105 cfu/ml. Total fungal count ranged from 5.8�±0.2��103 cfu/ml (sample A) to 4.0�±0.2��102 (sample D). The results of this study have shown that pond water if not properly checked could endanger both the fish and the potential consumers particularly if the fish harvested from these ponds are not properly cooked.