Micronuclei and other nuclear anomalies in exfoliated buccal mucosa cells of mexican women with cervical cancer

4^th World Congress on Cancer Science & Therapy

October 20-22, 2014 DoubleTree by Hilton Hotel Chicago-North Shore Conference Center, USA

Aurelio Flores-García, Jesús Salvador Velarde-Félix, Jorge Guillermo Sánchez Zazueta, María Luisa Ramos Ibarra, Eloy Alfonso Zepeda-Carrillo, Salvador Ruíz Bernés, Amelia Rodríguez-Trejo, Pedro Aguíar-García and Olivia Torres-Bugarín

Posters: J Cancer Sci Ther

Abstract :

Cervical cancer is the second most common cancer in women worldwide and the leading cause of cancer mortality in women in developing countries. Several studies have shown that the genome of primary cancer patients is unstable. The aim of the present study was to evaluate genomic instability in cervical cancer patients by means of the micronucleus (MN) assay. The frequencies of nuclear anomalies including micronuclei (MNi), binucleates (BN), and broken eggs (BE) were evaluated in exfoliated buccal mucosa cells (BMC) of Mexican women with primary cervical cancer and healthy women. A case-control study was performed in 40 never-treated patients in the State Cancer Center of Nayarit and 40 healthy females were used as the control group. Average age of participants was 47.07+8.21 and 47.67+8.04 years for cancer patients and control subjects, respectively. They signed an informed consent and were asked to complete a questionnaire concerning smoking habits, alcohol consumption, health status, diet and consumption of drugs or antioxidants. BMC were collected from each subject. A polished slide was used to collect cells from the buccal mucosa of the inner lining of both cheeks in each subject. Exfoliated cells were smeared on two slides. Smears were air-dried, fixed in 80% ethanol for 48 h and then stained with acridine orange. Cells were scored at 100x magnification using oil immersion with a fluorescent microscope. MNi andother anomalies (BN and BE) were evaluated in 2000 cells and were scored according to the criteria described by Thomas et al. and Bolognesi et al. Analysis of the data using the Mann-Whitney U-test showed that the frequencies of MN, BN and BE were significantly increased in cervical cancer patients compared with control group (p<0.0001). Cancer patients presented 2.82+0.98 MNi, 5.62+1.91 BN and 2.22+1.22 BE versus 0.82+0.26 MNi, 3.32+1.45 BN and 0.58+0.52 BE in control subjects. In conclussion, genomic instability was observed in BMC of Mexican women with cervical cancer.

Biography :

Aurelio Flores-García obtained his PhD in Immunology from the University of Guadalajara. He is currently a Professor and Researcher at the Autonomous University of Nayarit in the School of Medicine.