microRNA imaging in small animal model using the hNIS and the SPECT.CT modality :toward an imagedguided microRNAs radiotherapy?

International Conference on Nuclear Medicine & Radiation Therapy

July 14-15, 2016 Cologne, Germany

Patrick Baril

University of Orleans, Orleans, France

Posters & Accepted Abstracts: J Nucl Med Radiat Ther

Abstract :

miRNAs are key players of many biological processes and are considered as new class of pharmacology drugs for diagnostic and therapy. However to fully exploit the potential of miRNAs in the clinic, it is important to monitor their expression pattern using medical imaging modalities. Recently, we developed a method called RILES, for RNAi-Inducible Luciferase Expression System, that allows the monitoring of miRNA expression using bioluminescence equipment. Here we question whether changing the luciferase reporter gene by the human sodium iodide symporter (hNIS) as reporter gene will also permit to monitor expression of miRNAs using nuclear medicine imaging scanner. We provide the evidence that molecular imaging of miRNA expression using the hNIS and SPECT.CT (RINES system for RNAiinducible hNIS Expression system) is feasible but is not as robust as it is when the luciferase reporter gene is used. However under controlled conditions, we were able to monitor the expression of several miRNAs in cells and in the liver and tibialis anterior muscle of mice undergoing muscular experimental atrophy. We are currently exploiting the advantage of the non-invasive nature of the RINES to investigate for a possible link between the loss of miRNA- 23a expression, induction of the apoptosis and the rate of muscle mass degradation. Because the hNIS has a well-established dual application in radionuclide imaging and radiotherapy, our results warrant the use of the RINES for image-guided miRNA radiotherapy with possible application in personalizedmedicine.

Biography :

Email: Patrick.BARIL@cnrs.fr