miRNA alternation in adipose-derived stromal cells during differentiation towards nucleus pulposus cells

3^rd International Conference on Genomics & Pharmacogenomics

September 21-23, 2015 San Antonio, USA

Zhen Sun

Fourth Military Medical University, China

Posters-Accepted Abstracts: J Mol Genet Med

Abstract :

The application of stem cell therapy has made tremendous strides in intervertebral disc regeneration. Adipose-Derived Stromal Cells (ADSCs) were found to differentiate to Nucleus Pulposus (NP) cells following co-culture with NP cells. However, it remains unknown in the change of miRNA of the ADSCs during differentiation. Accordingly, the present study is aimed to clarify the miRNA alternation in ADSCs following co-cultured induction. Human ADSCs were co-cultured by Traswell with NP cells obtained from non-degenerate human NP tissue at 50:50 ratios for 7 days. Differentiation of ADSCs was measured and changes of miRNA in ADSCs were assessed by microarray. The ADSCs was showed to differentiate to the NP cell phenotype with an increased expression of related extracellular matrix. Importantly, it was found that 29 miRNAs were differentially expressed in the ADSCs following differentiation. The expression of miR-29a-5p, miR-4725-5p and miR-190a were up-regulated whereas miR- 802, miR-345-3p and miR-3201 were down-regulated (Fold Change>=2.0, p<0.05). In conclusion, this study provides an essential understanding and expands our knowledge of ADSCs differentiation in disc regeneration in miRNA level.

Biography :

Email: fmmusunzhen@126.com