Mitochondrial function, CBF and blood oxygenation monitored in vivo in gerbils under oxygen deprivation conditions

6^th International Conference and Exhibition on Biosensors & Bioelectronics

September 22-23, 2016 Phoenix, USA

Avraham Mayevsky

Bar-Ilan University, Israel

Posters & Accepted Abstracts: J Biosens Bioelectron

Abstract :

Normal mitochondrial function is a critical factor in maintaining cellular homeostasis in the brain as well as in various organs of the body. Due to the involvement of mitochondrial dysfunction in many pathophysiological conditions, realtime in vivo monitoring of the mitochondrial metabolic state is crucially important. This type of monitoring in animal models as well as in patients provides real-time data that can help to interpret experimental results or optimize patient treatment. The monitoring of mitochondrial NADH redox state in the brain provides the most important information on the metabolic state of the mitochondria in terms of energy production and intracellular oxygen levels. Nevertheless, monitoring of NADH alone is not sufficient for the understanding of brain function and more parameters must be measured in vivo. The aim of this study was to compare the effects of various situations, leading to oxygen deprivation, on cortical mitochondrial function, CBF and microcirculatory blood oxygenation to changes in systemic hemoglobin oxygenation measured by pulse oximetery. Mitochondrial NADH redox state was evaluated by surface fluorometry, CBF was evaluated by laser Doppler flowmetry and microcirculatoy hemoglobin oxygenation by two wavelength reflectometry. The various parameters were measured using a single optical fiber optic probe located and cemented to brain surface. Systemic blood oxygenation was measured by a special small animal adapted pulse oximeter attached to the leg of the gerbil. The models that were tested included systemic perturbation such as moderate hypoxia and anoxia as well as local ischemia induced by carotid artery occlusion in the gerbil (N=10). The results demonstrated that the mitochondrial NADH was the best indicator of intracellular energy balance. The responses of CBF to the various perturbations were not correlated to intracellular oxygen availability or consumption. Microcirculatory blood oxygenation was significantly correlated to CBF. Changes in systemic hemoglobin were recorded mainly under anoxia and hypoxia. In conclusion, these results demonstrated the potential use of mitochondrial NADH together with other hemodynamic parameters for the evaluation of brain viability under systemic (drug efficacy and safety) as well as focal perturbations.

Biography :

Email: mayevskya@gmail.com