Kui Luo
Zhengzhou University, China
Posters & Accepted Abstracts: J Med Microb Diagn
Background: Staphylococcus aureus (S. aureus) is an important human etiologic agent. Researching the characteristics
of popular genotype of S. aureus, relating to the pathogenicity and antibiotic resistance, can provide reference for
the prevention.
Methods: This study collected 275 S. aureus isolates from Zhengzhou city of China, including 148 isolates from
patients and 127 isolates from ready-to-eat food. Antimicrobial susceptibility testing was performed using the broth
dilution method. Molecular characteristics of antimicrobial resistance, virulence and genotypes were identified by
PCR.
Results: 34.18% (94/275) of S. aureus isolates were MRSA. Compared with food isolates, clinical isolates had
significantly higher antibiotic resistance rates, the carriage rates of resistance genes including acc(6')/aph(2'), aph(3')-
III, ermA, ermB and virulence genes including tetM, sea, seb, pvl and etb. MRSA-t030-agrII-SCCmeclll and MSSAt002-
agrII were the most common types among the clinical strains, and MRSA-t002-agrII-SCCmecIII and MSSAt002-
agrII among food strains. Besides, some strains found in agr group were also spa type-specific, suggesting that
there may be phenotypic consistency.
Conclusion: The clinical isolates contained more resistance genes and had higher antibiotic resistance, while the two
sources strains all had high toxicity. It indicates that the bacteria from different origins may have different evolution
process. Since resistance and virulence factors in food bacteria can be transmitted to humans, food handlers should
strictly follow hygienic measures during the process of food production in order to ensure the safety of human
consumers.
E-mail: luokui960002318@163.com
Medical Microbiology & Diagnosis received 14 citations as per Google Scholar report
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