Molecular cloning involving AAV-CXCL 12 gene

10^th International Conference and Exhibition on Biologics and Biosimilars

October 16-17, 2017 | San Francisco, USA

Kripa Raj Ahuja

The University of North Carolina, USA

Posters & Accepted Abstracts: J Bioanal Biomed

Abstract :

The American Cancer Society reports that this year there will be an estimated 600, 920 deaths due to cancer in the United States. Current cancer research includes the use of biomarkers on the surface of cancer cells to distinguish the cancerous cells from normal body cells. Molecular cloning can enhance these biomarkers. Over the past 30 years, molecular cloning has progressed immensely. From digestion to plasmid insertion, the possibilities are endless. The AAV (Adeno Associated Virus) CXCL 12 (C-X-C Motif Chemokine Ligand 12) is a Protein Coding gene that shows great promise with cloning and plasmid insertion. Our project aims to use this gene to bind tightly to biomarkers on the surface of cancer cells. However before this optimal binding can occur, it is essential to know more about the AAV CXCL 12 Gene itself. For this reason, our project includes multiple gel electrophoresis assays, plasmid insertion/digestion assays, and PCR purification. From the results of these assays, the efficacy of AAV CXCL 12 to bind to cancer biomarkers will become clear. In particular, the cloning assay for the AAV CXCL 12 gene holds great potential, as it is possible to clone extraneous DNA into a different host. If extraneous DNA can be cloned into a different host, then there is the possibility of that DNA binding to a biomarker on a cancer cell.

Biography :

Kripa Raj Ahuja is a sophomore at the University of North Carolina at Chapel Hill. She has been doing research for the past four years, since her sophomore year of high school. Over her research career, she has presented at various poster conferences and won a wide range of prizes. Currently, she is a Research Assistant at the Functional Proteomics Laboratory at the UNC Cancer Lineberger Center. Her research is centered on creation of a new nanotechnology that involves molecular cloning drug delivery mechanisms.