Molecular diversity of Staphylococcus aureus from the nares of diabetes mellitus patients in Yaounde, Cameroon

Agnes Bedie Eyoh; M Ehlers; R M Eliva; N J Mb; a; C Fokunang; M C Okomo; M Toukam; H Gonsu Kamga; E Lyonga; G Ikomey; M Mesembe; S Koulla-Shiro

doi:10.4172/2161-0703.C1.003

Molecular diversity of Staphylococcus aureus from the nares of diabetes mellitus patients in Yaounde, Cameroon

Global Medical Microbiology Summit & Expo

November 28-29, 2016 San Francisco, USA

Agnes Bedie Eyoh, M Ehlers, R M Eliva, N J Mbanda, C Fokunang, M C Okomo, M Toukam, H Gonsu Kamga, E Lyonga, G Ikomey, M Mesembe and S Koulla-Shiro

University of Yaound�?©, Cameroon
Center for the Study and Control of Communicable Diseases, Cameroon
University of Pretoria, South Africa

Posters & Accepted Abstracts: J Med Microb Diagn

Abstract :

Globally, information on the genotypic diversity of Staphylococcus aureus is relevant for managing the epidemiological and clinical challenges resulting from the clonal nature of this bacterium. The study objective was to determine epidemiological and clonal diversity of carrier Staphylococcus aureus isolates from the nares of diabetes mellitus patients in Yaounde, Cameroon. We enrolled 245 diabetes mellitus patients after obtaining assent. API-Staph was used to phenotypically identify isolates. Multiplex PCR assays were used for molecular confirmation, mecA (methicillin resistance) and the LukS/F-PV operon (Panton-Valentine Leukocidin). Multiplex PCR were also used for Staphylococcal cassette chromosome typing. Clonal relatedness was by Pulsed Field Gel Electrophoresis and genotyped isolates assigned sequence types by Multilocus Sequence Typing. 96 (39%) of participants were carriers of S. aureus. The mecA and lukS/F-PV genes were detected in 54% (52) and 58% (56) respectively. Simultaneously, 37% (36) carried the mecA and the lukS/F-PV genes. Overall, 56% (29) of the strains harboring mecA typed as SCCmec type V followed by type IV 38% (20). PFGE results clustered isolates into 14 pulsotypes. Based on MLST types, isolates were assigned to 6 pandemic clonal complexes (CCs): CC5 CC8, CC15, CC25, CC72 and CC121 and 3 atypical sequence types (ST 508, ST 699 and ST 1289) with ST 1289 having characteristics of epidemic clones. Prevalence of methicillin resistance and lukS/F-PV was high. S. aureus clones circulating in Yaounde are genetically diverse. These strains could have far reaching effects on epidemiology surveillance and control strategies in this context where genetic markers are not routinely monitored.