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Molecular identification of Mycobacterium tuberculosis transmission between cattle and man: A case report
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Veterinary Science & Technology

ISSN: 2157-7579

Open Access

Molecular identification of Mycobacterium tuberculosis transmission between cattle and man: A case report


Global Veterinary Summit

August 31- September 02, 2015 Orlando-FL, USA

Salisu Ibrahim

Ahmadu Bello University, Nigeria

Posters-Accepted Abstracts: J Veterinar Sci Technolo

Abstract :

The study describe a case of transmission of Mycobacterium tuberculosis (M. tb) infection from a man to cattle in a farm in Gombe State, North east, Nigeria. M. tb was isolated from the bronchial lymph nodes of a 2 year old heifer that reacted strongly positive to caudal fold intradermal tuberculin test but showed no gross pathological lesions at slaughter. The cattle attendant reported to hospital sick and was diagnosed smear sputum positive and he died at the time when the heifer was diagnosed as M. tb infected. The owner�s sputum and the heifer�s bronchial lymph nodes were all cultured on Lowenstein Jensen media containing both pyruvate and glycerol. The isolates obtained were Ziehl Neelsen stained in order to obtained smear positives. The smear positive isolates were then subjected to Bioline (SD TB Ag MPT64 Rapid) assay to differentiate them in to Mycobacterium tuberculosis complex (MTBC) and environmental Mycobacterium. The MTBC were further subjected to PCR technique using GenoType MTBC technique to differentiate them into their various species. It was found that both the isolates from the man as well as that of the heifer were M. tuberclosis species, it was then concluded that there was a possibility of an anthropozoonotic transmission. Further studies using VNTR and spoligotyping is herby suggested.

Biography :

Email:

sihadejia@gmail.com

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