Khlusov I A*, Litvinova L S, Shupletsova V V and Khlusova M Yu*
*Siberian State Medical University, Russia Immanuel Kant Baltic Federal University, Russia
Posters-Accepted Abstracts: J Cancer Sci Ther
We have examined response of human leukemic T-lymphoblastoid cells (Jurkat T-cells) to 24-h in vitro cultivation with titanium substrates (12*12*1 mm3) covered by titanium oxides (TiO, TiO2) bilateral coating was prepared by microarc method from an aqueous solution of 20 mass % orthophosphoric acid. 27-98 % of immortalized cells had CD3+CD4+CD71+CD45RA+ immunophenotype and secreted IL-2, IL-4, IL-8, IL-10 and TNF�±, but not IL-1b and IL-6. Other cell markers (CD8, CD16, CD56, CD25, CD95) were found at 0 - 2.5% of the cell population. Jurkat T-cells contact with titanium oxides coating reduced statistically CD3, CD4, CD8 and CD95 membrane markers presentation and decreased IL-4 and TNF�± secretion. Structural (antigens expression) and functional (cytokines secretion) inactivation of Jurkat T-cells was not connected with the generation of intracellular reactive oxygen species (ROS), and was not mediated by TiO2 nanoparticles (diameter of 14�±8 nm; doses of 1 mg/L or 10 mg/L). Spearmanâ��s correlation analysis showed the inhibiting action of the oxide surface roughness in the range of Ra=2.2-3.7 �¼m on the number of viable Jurkat T-cells (rs = - 0.95; n=9; p<0.0001) due to an elevating portion of necrotic forms in the cellular population, mainly. In turn, magnitude of negative electrostatic potential of the oxide surface rose linearly (r = 0.6; p<0.000001, n=60) with the Ra roughness index. The roughness of the titanium oxides coating induces its surface voltage that seems to promote morphofunctional suppression of tumor immune cells by electrostatic/biological mechanisms are not connected with intracellular ROS generation.
Email: Khlusov63@mail.ru
Cancer Science & Therapy received 5282 citations as per Google Scholar report
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