Croce MV and Ferretti VA
National University of La Plata, Argentina
Posters & Accepted Abstracts: J Cytol Histol
Introduction: MUC1/Muc1 mucin (MUC1 in humans and Muc1 in others species) is a transmembrane glycoprotein expressed at the apical surface of most epithelia. Studies on mouse and human embryos have established that Muc1/MUC1 expression coincides with the onset of epithelial sheet and glandular formation. Objective: Objective is to analyze temporal and spatial expression of Muc1 at different stages of rat development. Method: A total of 8 embryos of each gestational stage, 8 neonates and 8 adults were included. Embryonic organs studied were esophagus, stomach, small intestine, salivary gland, liver, pancreas, trachea, lung and kidney as well as embryonic and adult mammary gland. Results: By immunohistochemistry (IHC), Muc1 epithelial cell expression was observed in the stomach, lung and kidney at the gestational age of 13 days (13 D). In embryonic pancreas, Muc1 expression appeared at 14 D stage. In small intestine, a strong reaction was observed from 15 D while esophagus and trachea showed expression from 18 D. In the liver, a positive reaction was detected from 16 D while the ducts of the salivary glands showed reaction from 18D while at 20 D when mucous and serous acini were distinguished; Furthermore, Muc1 was observed in lactating mammary gland, post-lactational involution and old mammary gland. Lactating mammary gland showed an intense staining with a high percentage of reaction. In post-lactational involution and old mammary gland, a moderate reaction with a lower percentage of expression was detected. IHC results were validated by RT-PCR. Conclusion: Results suggested a possible role of Muc1 during epithelia formation, differentiation and glandular morphogenesis during mammary gland postnatal development.
Email: crocevir@hotmail.com
Journal of Cytology & Histology received 2476 citations as per Google Scholar report
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