Muscle transcriptome profiling of Kaupichthys hyoproroides

2^nd International Summit on Integrative Biology

August 04-05, 2014 Hilton-Chicago/Northbrook, Chicago, USA

Shaadi F Pooyaei Mehr and Wiebke Feindt

Accepted Abstracts: J Comput Sci Syst Biol

Abstract :

Ahigh ecological diversity and a great number of species organized in 20 families define the Anguilliformes. Recent research in eels mainly focuses on the critically endangered freshwater eel Anguilla anguilla investigating survival risks and stress response due to anthropogenic introduced water pollution (mainly pesticides) or in systematics in general. Little is known about other non-migratory eel species inhabiting coral reefs, and nothing in regards to their genomics. K. hyoproroides is an Atlantic circum-tropical species and some authors proposed to place it in synonymy with Indo-Pacific species Kaupichthys diodontus . Here we present the first high throughout transcriptome data of this circum-tropical marine living False Moray, Kaupichthys hyoproroides . Two individual high quality muscle RNA seq data sets were obtained using Illumina Hi-seq, 80- bp paired endreads. De novo assembly with two main de novo assembly programs has highlighted the assembly statistics in these two datasets. Comparative transcriptomic analyses between these two individuals have led to identification of similarly expressed genes (essential as calibration for future studies). Furthermore, comparisons at the order level with existing transcripotme data and EST?s of A. anguilla and A. japonica has shed light on order shared genes. Besides, these first annotated transcriptomes enable subsequent qualitative and quantitative studies in stress response and local adaptation using K. hyoproroides as ecologically important fish for anthropogenic reef destruction.