Nano-Liquid chromatography coupled MALDI MS/MS based quantitative cancer metabolomics

15^th International Conference on Metabolomics and Systems Biologys

August 29-30, 2019 | Vienna, Austria

Swarnendu Bag

Tata Medical Center, India

Posters & Accepted Abstracts: Metabolomics (Los Angels)

Abstract :

Tumour cells follow aberrant metabolic process for its survival and to escape from immune attack. Quantitative metabolomics is very crucial to understand the altered cancer micro-environment for targeted cancer theranostics. In this context, high-throughput separation and quantification of metabolites from clinical samples is an essential domain. Matrix Assisted Laser Desorption Ionization Mass Spectrometry (MALDI-MS), a gold standard technique for metabolite identifications, suffers from limitations in quantitation. In present work, a novel approach having nano-LC-MALDI MS/MS interface for separation, identification and label free quantifications of the metabolites from bio-samples has been evaluated. Metabolites extracted from cancer biopsies were separated and subsequently eluted as fraction spots on MALDI target plate. Introducing a novel parameter, % AAUC, quantification of metabolites isolated from cancer biopsies was explored. This comparative quantification is only possible with multiple AUCs of a particular metabolite obtained from this nano-LC-MALDI-MS. Selected metabolite peaks were analyzed through MS/MS fragmentation tool. This novel method was validated using known concentrations of an internal standard (thiourea). The novel quantification method revealed a significant change in expression of lipid metabotypes like phosphatidyl-choline, triglyceride, cytidine-diphosphate-diacylglycerol and phosphatidylinositol bisphosphate representing de-regulated lipid metabolism in oral cancer.