Raden Wasito, Hastari Wuryastuty, Ocie Harum Wulan and Niken Yunita
Gadjah Mada University, Indonesia
Ministry of Agriculture, Indonesia
Scientific Tracks Abstracts: J Vet Sci Technol
Avian Infl uenza (AI) and Newcastle Disease (ND) are two important viral diseases of poultry, which oft en lead to similar clinical signs and pathological lesions in poultry. Highly pathogenic AI and ND lead high morbidity and mortality, causing huge economic losses to the poultry industry. Confi rmatory diagnostics are necessary to distinguish between these two important viral diseases. Th e aims to this investigation was to assess the specifi city of two biotin-streptavidin immunohistochemistry tests (IHC-SB) to diff erentiate between infection caused by virulent Newcastle disease virus (vNDV) and highly pathogenic avian infl uenza virus (HPAI) in a commercial poultry farm. Th e laying hens showed clinical signs of torticollis and paralysis, as well as hemorrhagic lesions in the lungs and/or digestive system. Th e lungs, brains and digestive tract samples were collected tested with IHC-SB tests specifi c for either vNDV or HPAI. Th e results showed that these tests could be used to safety and reliably detect vNDV and HPAI-specifi c antigen in infected cells, thus confi rming their role in defi nitive diff erential diagnosis between these two pathogens.
Raden Wasito is a Professor of Veterinary Pathology at Department of Pathology, Faculty of Veterinary Medicine, Gadjah Mada University, Indonesia. He is currently a Staff Member at Faculty of Veterinary Medicine, Gadjah Mada University. He has received his DVM degree from Gadjah Mada University in 1978 and pursued his Advanced Degrees in Pathology at Department of Pathology, Michigan State University, USA and obtained MS in 1984 and PhD in 1987
Veterinary Science & Technology received 4472 citations as per Google Scholar report
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