Novel two-photon dyes: Minimal autofluorescence in tissue imaging

European Chemistry Congress

June 16-18, 2016 Rome, Italy

Juryang Bae, Hyunsoo Moon, Dokyoung Kim and Kyo Han Ahn

Pohang University of Science and Technology, Republic of Korea

Posters & Accepted Abstracts: Chem Sci J

Abstract :

The advantage of fluorescent imaging for biomaterials is operationally simple, cost-effective, noninvasive, high sensitive detection and visualization of organisms at a subcellular level. In tissue imaging, however, autofluorescence from biological molecules under excitation at UV�?¢�?�?�?�?Vis wavelengths lowers signal to noise ratio. Most of biomolecules absorb and emit light of green region. So, a novel class of fluorophores excited in the near-infrared region is necessary to suppress this critical issue. Two-photon absorbing dyes are one of the methods to satisfy this criterion. They have several advantages not only reduced autofluorescence, but also increased penetration depth, and high special-resolution. In addition, they diminish photodamage and photobleaching as well. Acedan, 6-acetyl-2-(dimethylamino) naphthalene, and its derivatives are widely used for twophoton dyes. But their maximum absorption wavelengths (~370 nm) are rather short for two-photon excitation (~740 nm) which shows strong autofluorescence and limits the depth in tissue in microscopic imaging of tissues. Herein, we have developed compact �?�?�?-extended acedan derivatives. They have the longer maximum absorption wavelengths more than 400 nm and sufficient two-photon absorption properties. One of the new dyes that can be excitable at 1000 nm under two photon excitation condition is photochemically stable and biocompatible. Also it has environment-sensitivity and readily penetrate the blood-brain barrier, allowing in vivo fluorescence imaging of A�?�?�?² plaques in a live mouse model of Alzheimer�?¢�?�?�?�?s disease.

Biography :

Juryang Bae received her BS from Pusan National University in 2013. Currently, she is a PhD candidate at Pohang University of Science and Technology (POSTECH).
Email:juryang@postech.ac.kr