Occurrence of Metallo-β-lactamase in clinical isolates of Escherichia coli and Klebsiella pneumoniae

International Conference on Medical and Clinical Microbiology

July 03-04, 2017 Bangkok, Thailand

Krishus Nepal

Tribhuvan University, Nepal

Posters & Accepted Abstracts: J Med Microb Diagn

Abstract :

Resistance mediated by Metallo-�²-Lactamases (MBL) enzyme to broad spectrum antibiotics is an increasing global problem. The member of Enterobacteriaceae producing MBL, particularly in E. coli and K. pneumoniae constitutes a serious threat to current �²-lactam therapy. Therefore, this study was conducted to know the occurrence of MBL producer in the clinical isolates of E. coli and K. pneumoniae. This was a descriptive cross sectional study conducted at OM Hospital and Research Centre (P) Ltd., Chabahil, Kathmandu, Nepal between May and December 2015. A total of 177 non-duplicate, consecutive isolates of E. coli (n=138) and K. pneumoniae (n=39) were isolated from different clinical samples. Of the total 177 isolates, 7 (4%) and 61 (34.5%) isolates were MBL and ESBL producer respectively. The co-existence of ESBL and MBL within the same isolates was not observed. MBL producing isolates showed high resistance to both �²-Lactam and non-�²-Lactam antibiotics used; entire MBL producers were Multi Drug Resistant (MDR). Of the drug used in the study, Colistin was only found effective for the treatment of MBL producing isolates. Our finding showed considerable number of MBL producing E. coli and K. pneumoniae isolates with MDR. Thus, it would be important for the early detection of MBL producing isolates for establishing potent antibiotics therapy which will be fruitful for controlling infection and also to avoid circulation of such strains within the hospital.

Biography :

Email: krishusnepal@gmail.com